Luminex xPONENT for MAGPIX User Manual

Page 79

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% Recovery - A measure of how accurately your observed results match your expected

results following regression analysis.

(Observed concentration) / (Expected concentration) x 100%

Expected Result - The known or expected test result value for a standard or control.

Control Range - Low - The lowest value for an assay control used to determine pass/fail

criteria for an assay.

Control Range - High - The highest value for an assay control used to determine pass/fail

criteria for an assay.

Normalized Net Median - For each analyte in a well the Normalized Net Median (NNM) =

(net median of analyte) / (net median of normalization bead)

Units - The unit of measure for an analyte, for example, pg/mL.

*Trimmed statistics remove the lower and upper five percent of the extreme statistic values,
then use the remaining values for the Mean, Standard Deviation, or % CV calculations.

The statistics displayed change according to the analysis type selected.

Analyte - Contains a list of analytes run in the batch. Select an analyte to view all statistics
for that analyte.

Current Well - Displays the statistics of the well currently being displayed (this changes to
Displayed Well if viewing a batch using the Open button of the Saved Batches tab).

Single Step - Allows analysis one well at a time. Click to turn this function on or off.

Results - Displays statistics related to the batch. Use the up, down, left, and right arrow
buttons to move through the table, or use the scroll bars. The Maximize/Minimize toggle icon
expands the batch table and returns it to the standard size.

Plate - A list of the available plates (if more than one).

CAUTION:

If using multiple plates, ensure that plates are used in the proper
order. Failure to do so can result in inaccurate data and test
results.

Right-click inside the dot plot area to access the following options:

Display Mode - Select either Logarithmic or Linear display. The software defaults to
Logarithmic display. Click maximize for a magnified view of the dot plot.

Log - Displays a log of system processes. The log includes the following information:

• Date

• Message

• Code

Log entries indicating warnings are highlighted yellow. Errors are highlighted red. Other log
entries are not highlighted. Click the Maximize icon to maximize the log. The log expands
to fill the whole window. Click the Minimize icon to return to the standard size.

Save Image - Opens a Save As dialog box to save a screen capture.

Details - Opens the Results tab, enabling more analysis and results.

Progress - Click to display counts per region of the selected well. Analyte counts are
displayed in a bar graph as they are acquired. The scroll bar at the bottom of the Progress
display scrolls through the analyte list.

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