Classification and reporter fluorochromes, Fluidics 1 and fluidics 2, Sample volume – Luminex xPONENT for MAGPIX User Manual

Page 19

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Classification and Reporter Fluorochromes

MagPix beads in the calibration kit are used to autofocus the camera and calibrate the CL1,
CL2, and RP1 channels. The beads in the verification kit are a mix of 6 different regions that
cover the range of the 50-plex map. Both calibration and verification beads are triple-dyed,
and the fluorescence signal of these dyes enables classification of each bead set.

TABLE 1.

MAGPIX Active Bead Regions (by Region)

Region

Region

Region

MC10012

MC10013

MC10014

MC10015

MC10018

MC10019

MC10020

MC10021

MC10022

MC10025

MC10026

MC10027

MC10028

MC10029

MC10030

MC10033

MC10034

MC10035

MC10036

MC10037

MC10038

MC10039

MC10042

MC10043

MC10044

MC10045

MC10046

MC10047

MC10048

MC10051

MC10052

MC10053

MC10054

MC10055

MC10056

MC10057

MC10061

MC10062

MC10063

MC10064

MC10065

MC10066

MC10067

MC10072

MC10073

MC10074

MC10075

MC10076

MC10077

MC10078

Fluidics 1 and Fluidics 2

Although it undergoes a wash step in between wells, the probe can be susceptible to carry-
over from well-to-well. Fluidics 1 contains one bead set. Fluidics 2 contains a buffer solution
and a different control bead. The function of this maintenance procedure is to measure how
much (as a percentage) of the first bead set in Fluidics 1 is found in the well where Fluidics 2
has been loaded.

Sample Volume

Sample volumes range in size from 20 µL to 200 µL. Ensure that approximately 25 µL more
than the sample volume remains in the well after aspiration. This amount may vary
depending on the type of plate used. Your sample volume must be large enough to prevent
aspirating air into the fluid line when acquiring sample, and small enough to prevent spill-over

xPONENT for MAGPIX
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