Luminex xPONENT for MAGPIX - Manual (Page 96)

12. In the Count box, type the total desired bead count for each analyte. Click Apply All.
13. To set a bead count and the units for a single analyte, click in the Units and Count
columns directly to the right of the analyte grid, and type a units value and bead count.
14. Click in the Call % column and type a value to set an individual analyte’s call percentage.
15. Click Next. The Plate Layout tab opens.
• To add well commands, highlight the appropriate wells and mark them as unknown,
standard, control, background, or wash. You can also delete commands that you’ve
added, and change the starting location on the plate. If you want to run in replicate,
change the Replicate Count to the appropriate number and the Grouping to your
preferred grouping method.
• Delete a command by clicking the well, and clicking Delete. The Delete Options
dialog box opens. Select Delete just the selected wells to delete a single well
command, or Delete all wells containing these samples to delete all wells with the
same command.
• As you add commands to your plate, they appear in the Command Sequence list.
Type a well ID in the ID field. Here, you can give each of your wells an ID. You can
also import a protocol file by clicking Import List.
• Move commands up or down in the sequence by highlighting a command and using
the Move Command arrows to move the command up or down in the list.
16. Click Save.
Importing a Protocol
1. Open the Protocols page, then open the Protocols tab. Click Import.
2. In the Open dialog box, navigate to the protocol file you want to import, then click Open.
3. The imported protocol displays in the Installed Protocols list.
Adding a New Lot for Protocol
1. Open the Protocols page, then open the Protocols tab. Click the protocol to which you
want to add a lot.
2. Open the Stds & Ctrls tab.
3. Click Create New Std/Ctrl Lots and select a protocol from the drop down list in the
Select Protocol dialog box, then click OK. The Std/Ctrl Details tab opens.
4. Click Apply Std/Ctrl Kit to associate a kit with the protocol. If you are not using a kit,
type the appropriate Standard and Controls information in the Assay Standard
Information and Assay Control Information sections.
5. Click Save.
Deleting a Protocol
1. Open the Protocols page, then open the Protocols tab.
2. Select a protocol.
3. Click Delete. The Delete Protocol dialog box opens.
4. Click Yes.
Using xPONENT
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