Leica Biosystems CM1520 - Manual (Page 32)

32 Instructions for Use V1.3 – 10/2012
7. Daily Use of the Instrument
7.2.1 Inserting the specimen discs into the
specimen head
7.2 Specimen discs
Fig. 24
• Lock the handle of the handwheel in the upper
position.
• If the knife holder and a knife are in place,
cover the knife edge with the knife guard.
• Loosen the clamping screw (1, Fig. 24 ) on the
specimen head.
• Insert the shaft of the specimen disc (3) in the
location hole (2) of the specimen head.
• Retighten clamping screw (1).
7.2.2 Specimen orientation
• Loosen the clamping lever (4).
• Orient the specimen on the ball shell using the
lever (5).
• Retighten the clamping lever (4).
7.1.2 Stationary heat extractor (optional)
• Fasten the support (1, Fig. 23 ) of the heat extractor (2, Fig. 23 ) to the left
side panel of the cryochamber using two screws in the provided holes
and insert the heat extractor.
• Lower the heat extractor cylinder on the specimen surface. After at least
30 seconds, the specimen is completely frozen.
• After the specimen is frozen, move the heat extractor into resting position
at the parking station (3, Fig. 23 ).
1
3
2
4
5
The specimen discs are to be handled
by their side O-rings to protect against
frostbite!
Make sure that the shaft of the speci-
men disc is fully inserted. The entire
surface of the rear side of the speci-
men disc must be free of residue.