Leica Biosystems CM1520 - Manual (Page 59)

59 Leica CM1520 – Cryostat
9.2 Spray disinfection with Leica Cryofect
9. Cleaning, Disinfection, Maintenance
For easy-to-use spray disinfection we recommend Leica Cryofect. (Leica Cryofect is not avail-
able in the USA!)
We recommend wearing the safety gloves provided in the standard scope of delivery
when disinfecting!
Exercise caution when cleaning the chamber – the plug in the rear on the left has sharp
edges and can lead to cutting injuries.
The cryostat, including all components, has to be disinfected after each daily use.
Comply with the instructions for use!
The glass anti-roll plate can remain in place during disinfection.
1. Set the cryochamber temperature up to a max. value of -20°C (the warmer, the better).
2. Remove the knife or blade from the knife/blade holder.
3. Remove all specimens, microscope slides and tools from the cryochamber.
4. Remove debris from the cryochamber.
Allow the cryochamber to reach the previously selected temperature.
Once the selected temperature is reached, either
5a. Spray the surfaces to be disinfected with a uniform coat of disinfectant (when using Leica
Cryofect, a maximum of 10 sprays are allowed - see 2.6. "Cleaning and disinfection" ) - while
doing so, the surfaces should be covered with a uniform coat of film - or
5b. soak a cloth with disinfectant and apply it on the contaminated surfaces.
6. In both cases, allow the disinfectant a reaction time of at least 15 minutes.
7. After the reaction time, wipe the disinfectant off using a tissue.
8. Dispose of the tissue according to applicable laboratory regulations.
9. Set the cryochamber temperature to the originally selected value.
If increased frost buildup occurs, start a manual defrost cycle.