System initialization – Luminex xPONENT 3.1 Rev 2 User Manual
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11. Tighten the probe adjustment screw.
NOTE: Use caution not to move the probe up accidently when tightening the
probe adjustment screw.
12. Click Move Probe Up.
13. Replace the plastic shield that covers the sample probe area.
14. Click Eject to eject the plate holder. Remove the alignment tools from the plate.
System Initialization
xPONENT
®
Software contains a pre-defined startup routine to prepare the analyzer for data
acquisition. This section describes warmup, calibration, and performance verification of the
system.
Warm up the lasers to prepare the optics prior to sample acquisition. The system
automatically begins warming up when you turn power on; however, you will need to use the
Warmup command if the system is idle for four hours or longer. Failure to properly warm up
the lasers will affect assay results and system performance.
• On the System Status bar, click the Laser: button if it is yellow.
or
• Open the Maintenance page, and then the Cmds & Routines tab. Click Warmup in the
Commands section at the left of the screen, and then click the Run button at the bottom
right to begin the procedure. The warmup process takes 30 minutes to complete.
Calibrator xMAP beads are used to normalize the settings for the reporter channel, both
classification channels, and the doublet discriminator channel. Verification xMAP beads are
used to verify calibration and optical integrity of the system.
NOTE: When you run MagPlex microspheres, it is essential to use both
regular calibration reagents (CAL1 and CAL2) and magnetic
calibration reagents (MagCAL1) every time the system is calibrated.
Both must pass for the system to be fully calibrated and ready for
running magnetic beads.
Once calibrated, the values remain until you recalibrate. You can track system calibration and
verification results through the Calibration report. Target value information for calibration and
verification beads is available on the Luminex website at
.
Calibrate your system at least once a week using the Calibration/Verification button on the
Auto Maint tab of the Maintenance page. In addition, recalibrate the system if the delta cal
temperature exceeds +/-3º C. Verify the system daily using the Performance Verification
button on the Auto Maint tab of the Maintenance page. Refer to your assay kit instructions
for additional calibration frequency requirements.
Alternatively, you can set up the system initialization routine to include calibration and or
verification under the Admin page, System Setup tab, Maintenance Options section.
Ensure that the Luminex analyzer lasers are warmed up and the probe height is set correctly
before calibrating the system. Do not move the system waste line while calibrating.
Using the Software
97