Luminex xPONENT 3.1 Rev 2 - Manual (Page 124)

9. Check Mark as Intra-Well Normalization Bead if you want to use a normalization bead.
The normalization bead is a microsphere set that is included in the assay as an internal
control. It controls for sample variation and can be used to normalize data between
samples in a run. An analyte used as a normalization bead will appear blue in the analyte
grid.
10. Type a name for the new formula in the Formula Name box.
11. Click Save Formula. The new formula displays in the Formulas list.
12. Click Apply to All Analytes to apply the new formula to all analytes in the list, or click
OK to apply the new formula to the single analyte you first clicked.
Creating a Quantitative Assay Protocol
The protocol must contain multiple standards. The standards are assigned lot value
information for each test. A standard curve is generated according to the lot values. Including
controls in the protocol is optional, but recommended for judging the acceptability of batch
results.
To create a quantitative assay protocol:
1. Open the Protocols page, then open the Protocols tab. Click Create New Protocol.
The Settings tab opens.
2. In the Name box, type the name of the protocol.
3. Type a description in the Enter optional description here box.
4. In the Version box, type the version of the protocol.
5. In the Manufacturer box, type the manufacturer information for the protocol.
6. Define settings in the Acquisition Settings section.
7. Define settings in the Analysis Settings section, selecting Quantitative as the analysis
type. The protocol for a quantitative assay must contain multiple standards. Controls are
optional in a quantitative assay protocol.
8. Click Next. The Analytes tab opens.
9. Click the desired analytes (bead ID) in the numbered analyte grid. Information about the
analyte displays to the right side of the grid.
10. Click and type an analyte name in the Name column to the right of the analyte grid.
11. Click and type the desired unit of measurement in the Units box to the left of the Apply
All button.
12. Click and type the desired bead count for each analyte in the Count box. Click Apply All.
13. To set a bead count and the units for a single analyte, click in the Units and Count
columns directly to the right of the analyte grid, and type a bead count and units value.
14. To change the default analysis for all analytes, click Change. The Analysis Settings
dialog box opens.
15. In the Analysis Settings dialog box, select the analysis method from the Method list,
and the weighting in the Weight Type list. Click Apply to All Analytes to apply the
selection to all analytes.
16. To change the analysis for a single analyte, click the Analysis field for the analyte you
want to modify. The Analysis Settings dialog box opens.
17. Select an analysis method in the Method list.
Using the Software
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