4 viability, 5 labbook – Multichannel Systems Cardio2D Manual User Manual
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Cardio 2D Software
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4.4
Viability
Click Viability. The "Viability" icon
changes into "Stop" icon
, and the duration of
the viability test is counted up in hours, minutes and seconds. Stop the viability test manually by
clicking on "Stop" icon.
Running a viability test
Before you start recording, a viability test is recommended. The data is not recorded.
The viability test monitors whether the MEA electrodes are acceptable for recording, and the
cell culture or tissue is fine. You can check the signal detection parameters, and focus onto single
channels. If the settings are too rigid the cardiac signal is not detected at all. Too loose settings
will lead to a wrong signal detection, and thus to wrong results.
After adjusting all parameters in the different window panes you can start recording. The log file
documents the experimental proceeding.
It is possible to change analysis parameter during viability test is running to check the differences
immediately. This option is not available during recording, the settings have to be constant during
the whole time of recording.
4.5
Labbook
Click "Labbook" icon
. The dialog "Labbook Settings" will open. Click "Study" tab.
In this dialog you fill in data referring to your experiment. The "Labbook Settings" will be stored
in the settings file *.c2s.
Comparing "Local" and "Global" Settings
If more than one MEA is connected to the experimental setup or a multi well MEA you can control
each MEA or each well separately via "local" settings or you can control all MEAs together via
"global" settings. Please use the "Labbook" icon in the MEA window for "local" settings and the
icon in the "Control View" for global settings. Please use the "global" settings which apply to all
available MEAs or wells as well whereas changes in local settings will apply to a single MEA or a
single well. Changes in global settings will of course overwrite local settings. If local and global
settings are different, the respective input field will be highlighted in orange.