Multichannel Systems Cardio2D Manual - Manual (Page 51)

Cardio 2D Software
47
Analyzer Settings
Click "Analyze" icon . The "Analyzer Settings" dialog will open. Click "HB Detection" tab.
As in the "Labbook" settings the same is in "Analyzer" settings: You can have local settings on
each MEA or each well of a MEA or you control all MEAs at a time via global settings. The global
settings (white background) overwrite the local settings (orange background).
In this dialog you define the parameter for detecting the waveform of a field potential.
Please see also "Automated Waveform Detection" and "Detection Parameter".
The feature "Comparing "Local" and "Global" Settings" is also available for this dialog.
Please read chapter "Labbook".
The first parameter for detection is the "Rise Time". Select the minimum and the maximum
of the rise time from the drop down menus. The range involves 100 μs to 2 ms for the minimum
rise time, and 200 μs to 5 ms for the maximum rise time.
Next parameter is the "Amplitude" You can decide to have a fixed amplitude for detection
or a signal dependent amplitude. The fixed amplitude involves a voltage range from -10 μV
up to -1 mV. Using the signal dependent amplitude, the waveform detection is more flexible:
The detection routine detects the amplitude in relationship to the background noise. The
amplitude is calculated by the standard deviation of the signal times the factor you can choose.
It is recommended to use a signal dependent amplitude.
The following parameters characterize the detected field potential. "Detection Deadtime"
defines the time in which no detection is performed.