Multichannel Systems Cardio2D Manual - Manual (Page 53)

Cardio 2D Software
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Click "2D" tab in Analyzer Settings.
In this dialog you can define the time span from the drop down menu in a range of 1 to 200 ms
in which the "Propagation Time" should be calculated. The action potential runs over the heart
cell culture or tissue or whole heart from the pacemaker to the peripheral regions of the
syncytium. The excitation propagation is measured in high spatial resolution by the MEA
electrodes detecting cardiac field potentials and extracting local activation times. The propagation
time is the longest possible period for passing of the excitation from the first detected excited
electrode to the last detected excited electrode of one cluster. If the propagation time is too
short, you miss data for the complete heartbeat. If the propagation time is too long, the next
heartbeat starts and the results are incorrect. So, the appropriate time has to be tested empirical,
depending on the experiment.
The "Min Cluster Channels" gives the minimum number of channels at which heartbeats have
to be detected to form a cluster. For example, if a syncytium covers all 60 electrodes of a MEA
it could be 10, otherwise it should be less than 10.