Recommended volumes, 3 recommended volumes – YSI 5200 User Manual

Page 63

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YSI Incorporated

61

5200 Recirculating System Monitor

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If you are using laboratory glassware for calibration, you do not need to remove the probe sensor
guard to rinse and dry the sensors between calibration solutions. The inaccuracy resulting from
simply rinsing the sensor compartment and drying the outside of the guard is minimal.

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If you are using laboratory glassware, remove the stainless steel weight from the bottom of the
YSI 5562 Probe Module sensor guard by turning the weight counterclockwise. When the weight
is removed, the calibration solutions have access to the sensors without displacing a lot of fluid.
This also reduces the amount of liquid that is carried between calibrations.

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Make certain that port plugs are installed in all ports where sensors are not installed. It is
extremely important to keep these electrical connectors dry.

6.1.3 Recommended

Volumes

Follow these instructions to use the calibration/storage cup for calibration procedures.

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Ensure that an o-ring is installed in the o-ring groove of the calibration/storage cup bottom cap,
and that the bottom cap is securely tightened.

NOTE: Do not over-tighten as this could cause damage to the threaded portions.

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Remove the probe sensor guard, if it is installed.

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Remove the o-ring, if installed, from the probe module and inspect the installed o-ring on the
probe module for obvious defects and, if necessary, replace it with the extra o-ring supplied.

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Some calibrations can be accomplished with the probe module upright or upside down. A
separate clamp and stand, such as a ring stand, is required to support the probe module in the
inverted position.

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To calibrate, follow the procedures in the next section, 6.2 Calibration Procedures. The
approximate volumes of the reagents are specified below for both the upright and upside down
orientations.

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When using the Calibration/Storage Cup for dissolved oxygen % saturation calibration, make
certain that the vessel is vented to the atmosphere (by loosening the bottom cap or cup assembly)
and contains approximately 1/8” of water.

Sensor to Calibrate

Upright

Upside Down

Conductivity 55ml

55ml

pH, pH/ORP

30ml

60ml

Table 6-1 Calibration Volumes

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