Operation – Leica Biosystems SM2500 User Manual

Page 28

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5.4.5 Area 2.2 - Section/milling thickness, section/milling stroke preselection, retraction

10 SET (button)

Press SET to activate indication of FEED, n or RETRACT: LED starts emit-
ting light.
For entering new parameters with selector switch (9), press and hold
SET until the corresponding LED starts blinking.

11 FEED (LED)

When LED FEED is blinking: rotate switch (9) to enter a section/milling thick-
ness from 0 μm (knife / miller feed off) to 1,000 μm (selectable in 1-μm steps).

12 n (LED)

When LED n is blinking and PROG mode is activated: rotate switch (9) to
preselect the number of sections / milling strokes to be carried out (from
1 to 1,000).

13 RETRACT (LED)

When LED RETRACT is blinking: rotate switch (9) to enter a setting between
0 μm (retraction switched off) to 1,000 μm (selectable in 1-μm steps).

In AUTO and PROG mode, the knife / miller is lifted away from the
specimen surface at the end of each section / milling stroke (retrac-
tion).
The knife / miller then stays in retraction position over the entire
length of the sledge return stroke.
When the retraction feature is activated (retraction value >0),
RETRACT in area 5.2 emits light while the sledge is in return stroke.
When the sledge reaches the front end position, the knife / miller is
lowered back towards the specimen by the selected retraction value.

14 Indication of section/milling thickness, section/milling stroke

preselection or retraction parameters that have been entered via rotary
switch (9). - All parameters are displayed flush right.

All parameters are stored automatically upon entry (no confirma-
tion necessary) and remain stored until new settings are entered.
The LEDs stop blinking automatically after a few seconds.

14

12

10

13

11

5.

Operation

Fig. 18

28

Instruction manual V 1.3 – 10/2007

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