3 sectioning, Sectioning, Operation – Leica Biosystems SM2500 User Manual
Page 62
62
Instruction manual V 1.3 – 10/2007
5.6.3 Sectioning
Sectioning can be done in modes of operation AUTO or PROG.
Sectioning in AUTO mode of operation:
Preparatory steps:
As described for trimming (see page 59).
•
Select section thickness and speed according to the type of specimen being
sectioned.
•
Select a sectioning mode (see chapter 5.4.6). Sectioning can be done in
either CONT, SINGLE or STEP mode of operation.
Starting sectioning:
•
Press RUN STOP + RUN ENABLE or press the foot switch.
•
In SINGLE mode, the instrument will carry out one single step and then
stop in the stop or photo position selected.
•
In STEP mode, the instrument will continue sectioning as long as RUN
STOP + RUN ENABLE or the foot switch are being held.
•
To stop sectioning in CONT mode:
Press RUN STOP or RUN ENABLE or press the foot switch once again.
Sectioning in PROG mode:
Preparatory steps:
Proceed as described for trimming.
•
If necessary, select sectioning thickness and speed appropriate for the type
of specimen being sectioned.
•
Enter the desired number of sections ‘n’ you wish to be carried out (area
2.2 chapter 5.4.5).
Start sectioning:
•
Press RUN STOP + RUN ENABLE or press the foot switch:
The instrument will carry out the number of sections programmed and
then stop in the defined stop or photo position.
5.
Operation