6 mounting the knife holders, 7 inserting knives into the holders, Mounting the knife holders – Leica Biosystems SM2500 User Manual

Page 46: Inserting knives into the holders, Operation

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46

Instruction manual V 1.3 – 10/2007

5.5.6 Mounting the knife holders

Handle microtome knives and blades with utmost care. Knife and
blade edges are extremely sharp and can cause severe injuries!

Wear cut-resistant safety gloves when inserting / removing the
knife (see also chapter 8.1 ‘Additional accessories’).

Never leave knives / disposable blades or knife holders with a
knife / disposable blade inserted lying around!

Always store knives in the knife case when not in use!

Never place a knife on a bench with the edge facing upwards!

Never try to catch a falling knife or disposable blade!

Always clamp the specimen first and the knife second!

5.5.7 Inserting knives into the holders

For a detailed overview on which types of knives are appropriate
for which knife holder, please refer also to chapter 8.2 on page 66.

The knives are inserted into the different knife holders as follows:

Knife holder A:

Clamp the knives with the writing facing upwards, i.e. with the wedge angle
pointing downward, towards the specimen surface.

Knife holder C:

Clamp the knives with the writing facing upwards.

Knife holder B:

c-profile knives may be clamped with either surface facing upwards.
b-profile knives must be clamped with the facet pointing backwards when
inserting the knife, i.e. pointing away from the specimen once the knife has
been turned into sectioning position.

5.

Operation

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