Bio-Rad Criterion™ TBE-Urea Precast Gels - Manual (Page 11)

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2.2 Required Materials
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Criterion ™ precast gels
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Criterion or Criterion ™ Dodeca ™ cell
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PowerPac ™ Basic or PowerPac HC power supply (or equivalent)
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Sample buffer
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Running buffer (460 ml per gel)
2.3 Setting Up and Running Criterion Gels in the Criterion Cell
1. Each Criterion gel is packaged in a plastic storage tray. Remove the cover of the tray by lifting the
corner tab and pulling it diagonally across the package. Remove the gel from the package.
2. Remove the comb and rinse the wells with deionized water (diH
2
O) or running buffer.
3. Remove the tape from the bottom of the cassette by pulling the tab across the gel.
4. Insert the cassette into one of the slots in the Criterion cell tank so that the integrated upper buffer
chamber faces the center of the cell (A).
Integrated upper buffer
chamber
5. Fill each integrated upper buffer chamber with 60 ml
running buffer.
6. Fill each half of the lower buffer tank with 400 ml
running buffer to the marked fill line.
7. Load samples using a syringe or a pipet with
gel-loading tips.
Optional: place a sample loading guide on the
outer edge of the cassette to help align pipet tips
with the wells (this is particularly useful when using
multichannel pipets).
8. Place the lid on the tank, aligning the color-coded
banana plugs with corresponding jacks on the lid.
See Chapters 3–10 for power supply settings.
2.4 Removing the Gel
1. After electrophoresis is complete, turn off the power
supply and disconnect the electrical leads.
2. Remove the lid from the tank and remove the
gel(s) from the cell. Pour off and discard the upper
running buffer.
3. Invert the cassette and place the integral buffer
chamber over the cassette-opening tool built into
the Criterion cell lid (B).
A
B
Instruction Manual and Application Guide