Bio-Rad Criterion™ TBE-Urea Precast Gels - Manual (Page 14)

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Table 3.1. Criterion precast gels for SDS-PAGE.
Gel Formulation Gels Description
TGX (Laemmli-like) Criterion ™ TGX ™ Laemmli-like, extended shelf life gels
Criterion ™ TGX Stain-Free ™ Laemmli-like, extended shelf life gels with trihalo compounds for rapid
fluorescence detection
Tris-HCl (Laemmli) Criterion Tris-HCl Tris-HCl Laemmli gels
Criterion Stain Free ™ Tris-HCl Laemmli gels with trihalo compounds for rapid fluorescence
detection
Bis-Tris Criterion ™ XT Bis-Tris Based on a Bis-Tris HCl buffer system (pH 6.4); use these gels with
Criterion XT MES buffer for optimum resolution of small proteins
Bis-Tris Criterion XT Bis-Tris Based on a Bis-Tris HCl buffer system (pH 6.4); use these gels with
Criterion XT MOPS buffer for optimum resolution of midsized proteins
Tris-acetate Criterion XT Tris-acetate Based on a Tris-acetate buffer system (pH 7.0)
3.2.1 Criterion TGX and Criterion TGX Stain-Free Gels
Criterion TGX (Tris-Glycine eXtended shelf life) gels are Laemmli-like gels with a proprietary modification
that extends their shelf life to 12 months and enhances separation characteristics relative to conventional
gel types. The TGX formulation yields Laemmli-like separation patterns with short run times and
exceptionally straight lanes and sharp bands. TGX gels offer excellent staining quality, greater transfer
efficiency, and molecular weight estimation without the need for special, expensive buffers.
These gels are run using standard Laemmli sample buffer and Tris/glycine/SDS running buffer. Two
types of TGX formulations are available:
n
Criterion TGX — Laemmli-like, extended shelf life gels
n
Criterion TGX Stain-Free — Laemmli-like, extended shelf life gels with trihalo compounds
that allow rapid fluorescent detection of proteins with the Criterion Stain Free system,
eliminating staining and destaining steps for faster results (see Chapter 5 for more details)
Gel Composition
Crosslinker 2.6% C
Stacking gel 4% T, 2.6% C
Shelf life ~12 months at 2–8°C; expiration date is printed on each cassette
Gel Percentage Optimum Separation Range
7.5% 40–200 kD
10% 30–150 kD
12% 20–120 kD
18% 10–50 kD
4–15% 20–250 kD
4–20% 10–200 kD
8–16% 20–120 kD
10–20% 10–100 kD
Any kD ™ 1 10–200 kD
1 Any kD is a unique single-percentage formulation that provides a broad separation range and short running time.
Criterion Precast Gels