Bio-Rad Criterion™ TBE-Urea Precast Gels - Manual (Page 32)

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9.4 Sample Preparation
Determine the DNA concentration of your sample based on the detection method used. (See Chapter
12 for approximate stain sensitivities.) Dilute 4 parts sample with 1 part sample buffer.
9.5 Running Conditions
Table 9.1. Running conditions for nondenaturing nucleic acid PAGE with Criterion gels in the Criterion cell.
5% and 10% Gels 15% and 4–20% Gels
Power conditions 100 V constant 150 V constant
Expected current (per gel)
Initial 20–25 mA 27–35 mA
Final 14–18 mA 20–35 mA
Run time 90 min 90 min
Criterion Precast Gels