Bio-Rad Criterion™ TBE-Urea Precast Gels - Manual (Page 24)

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5.2 Criterion Stain Free Workflow
Analyze the Separation
Perform Electrophoresis
SDS-PAGE (Chapter 3)
Native PAGE (Chapter 4)
2-D Electrophoresis (Chapter 11)
Stain the Gels for Total Protein
(Chapter 12)
Blot the Gels
(Chapter 13)
Activate/Image Gels
(Chapter 5)
5.3 Electrophoresis with Criterion TGX Stain-Free Gels
Criterion TGX Stain-Free gels (and Criterion Stain Free gels) are made and packaged without SDS, so
they can be used for both SDS and native PAGE applications. To perform electrophoresis with these
gels, prepare the sample and running buffers, set up the Criterion cell, and perform the run as directed
in Chapters 2–4.
Use unstained standards with Criterion TGX Stain-Free and Criterion Stain Free gels,
as some prestained standards are not detected by Stain-Free technology.
To monitor electrophoresis, use 10 µl of a 1:1 mixture of Precision Plus Protein
™ unstained
and Precision Plus Protein All Blue protein standards.
5.4 Using the Gel Doc EZ Imager
Image Criterion TGX Stain-Free and Criterion Stain Free gels and blots in the Gel Doc EZ imager.
The imager activates the reaction between the proteins and trihalo compounds in the gel to enable
visualization.
n
Immediately place the gel in the tray of the imager; no fixation or rinsing steps are required.
Prolonged rinsing may diminish image quality and lead to gel deformation
n
If desired, stain the gel with any TGX-compatible stains after imaging. Certain stains, if used
prior to imaging, eliminate detection capability
Refer to the Gel Doc EZ Stain-Free Sample Tray Instruction Manual (bulletin 10019634) for detailed
instructions.
Criterion Precast Gels