5 running conditions – Bio-Rad Criterion™ TBE-Urea Precast Gels User Manual

Page 34

Advertising
background image

28

Technical Support: 1-800-4BIORAD • 1-800-424-6723 • www.bio-rad.com

10.5 Running Conditions

Table 10.1. Running conditions for denaturing nucleic acid PAGE with Criterion gels in the Criterion cell.

5% Gels

10% Gels

15% Gels

Power conditions

200 V constant

200 V constant

200 V constant

Expected current (per gel)

Initial

40–45 mA

20–33 mA

18–22 mA

Final

20–25 mA

14–18 mA

10–15 mA

Run time

90 min

90 min

90 min

Criterion Precast Gels

Advertising