5 storage conditions, 6 important notes – Bio-Rad Criterion™ TBE-Urea Precast Gels User Manual

Page 9

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1.5 Storage Conditions

Table 1.2. Storage conditions for Criterion precast gels. Store gels flat. Shelf life is from date of manufacture; expiration dates
are printed on the cassettes.

Storage Temperature

Gel Formulation

Shelf Life

Ambient

Criterion XT Bis-Tris

12 months

2–8°C

Criterion TGX

12 months

Criterion TGX Stain-Free

12 months

Criterion Tris-HCl

12 weeks

Criterion Stain-Free

12 weeks

Criterion XT Tris-acetate

8 months

Criterion Tris-Tricine

12 weeks

Criterion IEF

24 weeks

Criterion zymogram

8 weeks

Criterion TBE

12 weeks

Criterion TBE-urea

8 weeks

1.6 Important Notes

Use each Criterion precast gel as soon as possible after removing it from the storage pouch.

Improper storage of Criterion precast gels can produce numerous artifacts.

n

Store gels flat

n

Avoid prolonged storage at temperatures above those recommended

n

Do not freeze gels

n

If you suspect your gels have been stored improperly, discard them

Do not run more than one gel type in the same apparatus at the same time. Different gel percentages
and formulations have different conductivities and different run times.

Use unstained standards with Criterion TGX Stain-Free and Criterion Stain Free gels, as some
prestained standards are not detected by the Gel Doc

EZ imager. To monitor electrophoresis, use

10 µl of a 1:1 mixture of Precision Plus Protein

unstained and prestained standards.

Instruction Manual and Application Guide

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