Bio-Rad Criterion™ TBE-Urea Precast Gels - Manual (Page 30)

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8.4 Sample Preparation
1. Determine the appropriate protein concentration of your sample based on the detection method
and load volume used. (See Chapter 12 for approximate stain sensitivities.)
2. Dilute 1 part sample with 1 part sample buffer. Do not heat the samples.
8.5 Running Conditions
Power conditions 125 V constant
Starting current 90–120 mA/gel
Final current 35–55 mA/gel
Run time 90 min
Criterion Precast Gels