Bio-Rad Criterion™ TBE-Urea Precast Gels - Manual (Page 26)

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6.4 Sample Preparation
1. Determine the appropriate concentration of sample to load (depends on the load volume and the
detection method used; see Chapter 12 for approximate stain sensitivities).
2. Dilute the sample with at least an equivalent volume of sample buffer (catalog #161-0739) and
reducing agent ( β-mercaptoethanol, for example). Heat the diluted sample at 90–95°C for 5 min,
or at 70°C for 10 min.
For example, combine: 5 μl sample
4.75 μl Tricine sample buffer (catalog #161-0739)
0.25 μl β-mercaptoethanol (catalog #161-0710)
10 μl total volume
6.5 Running Conditions
Power conditions 125 V constant
Starting current 140–150 mA/gel
Final current 60–70 mA/gel
Run time 120 min
Criterion Precast Gels