Bio-Rad Criterion™ TBE-Urea Precast Gels - Manual (Page 15)

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3.2.2 Criterion Tris-HCl and Criterion Stain Free Gels
These Tris-HCl, Laemmli gels use discontinuous glycinate and chloride ion fronts to form moving
boundaries to stack and then separate denatured proteins by size. They are run using standard Laemmli
sample buffer and Tris/glycine/SDS running buffer.
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Criterion Tris-HCl — Tris-HCl formulation that offer a shelf life of 12 weeks
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Criterion Stain Free — Tris-HCl gels with unique trihalo compounds that allow rapid
fluorescent detection of proteins with the Criterion Stain Free system, eliminating staining
and destaining steps for faster results (see Chapter 5 for more details)
Gel Composition
Gel buffer 0.375 M Tris-HCl, pH 8.6
Crosslinker 2.6% C
Stacking gel 4% T, 2.6% C
Storage buffer 0.375 M Tris-HCl, pH 8.6
Shelf life ~12 weeks at 2–8°C; expiration date is printed on each cassette
Gel Percentage Optimum Separation Range
5 % 100–250 kD
7.5% 40–200 kD
10% 30–150 kD
12.5% 20–120 kD
15% 10–100 kD
18% 10–50 kD
4–15% 20–250 kD
4–20% 10–200 kD
8–16% 20–120 kD
10–20% 10–100 kD
10.5–14% 25–200 kD
Instruction Manual and Application Guide