Bio-Rad Criterion™ TBE-Urea Precast Gels - Manual (Page 46)

Quick Start Guide
A
Running buffer (1x) Dilute 100 ml 10x stock (catalog #161-0732) with 900 ml diH
2
O.
Sample buffer Use Laemmli sample buffer (catalog #161-0737).
Prepare Buffers
Prepare and Load Samples
Prepare Gels and
Assemble Electrophoresis Cell
Perform Electrophoresis
Remove the comb and tape from the gels and assemble the electrophoresis cell.
Fill the inner and outer buffer chambers with running buffer.
Fill the lower buffer chamber with 400 ml running buffer (to the fill mark).
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The following instructions are for electrophoresis of Criterion ™ precast gels using the Criterion system.
Component Reducing Nonreducing
Sample 5 μl 5 μl
Laemmli sample buffer
(catalog #161-0737) 4.75 μl 5 μl
β-Mercaptoethanol 0.25 μl —
Total volume 10 μl 10 μl
Heat samples at 90–100°C for 5 min or at 70°C for 10 min.
Load the appropriate amount of sample on the gel.
Connect the electrophoresis cell to the power supply and perform electrophoresis according to
the conditions in Table A.1.