Bio-Rad Criterion™ TBE-Urea Precast Gels - Manual (Page 21)

See Appendix B for buffer formulations. Do not adjust pH unless instructed to do so.
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Gel Composition
Crosslinker 2.6% C
Stacking gel 4% T, 2.6% C
Shelf life ~12 months at 2–8°C; expiration date is printed on each cassette
4.2.2 Criterion Tris-HCl and Criterion Stain Free Gels
These Tris-HCl Laemmli gels are run using native sample buffer and Tris/glycine running buffer.
n
Criterion Tris-HCl — Tris-HCl, Laemmli-like formulation that offer a shelf life of 12 weeks
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Criterion Stain Free — Tris-HCl, Laemmli-like gels with unique trihalo compounds that allow
rapid fluorescent detection of proteins with the Criterion Stain Free system, eliminating
staining and destaining steps for faster results (see Chapter 5 for more details)
Gel Composition
Gel buffer 0.375 M Tris-HCl, pH 8.6
Crosslinker 2.6% C
Stacking gel 4% T, 2.6% C
Storage buffer 0.375 M Tris-HCl, pH 8.6
Shelf life ~12 weeks at 2–8°C; expiration date is printed on each cassette
4.2.3 Criterion XT Tris-Acetate Gels
Criterion XT Tris-acetate gels can also be used to separate proteins by their charge-to-mass ratio
(under native PAGE conditions). Separation by native PAGE with XT Tris-acetate gels uses discontinuous
acetate and Tricine ion fronts to form moving boundaries to stack and separate proteins by both size
and charge.
Gel Composition
Gel buffer Tris-acetate, pH 7.0
Crosslinker 3.8% C
Stacking gel 4% T, 3.8% C
Storage buffer Tris-acetate, pH 7.0
Shelf life 8 months at 2–8°C; expiration
date is printed on each cassette
4.3 Native PAGE Buffers
Running buffer (1x) 25 mM Tris, 192 mM glycine
Dilute 100 ml 10x stock (catalog #161-0734) with 900 ml diH
2
O
Sample buffer 62.5 mM Tris-HCl, pH 6.8, 25% glycerol, 0.01% bromophenol blue
(catalog #161-0738)
Instruction Manual and Application Guide