Bio-Rad Criterion™ TBE-Urea Precast Gels - Manual (Page 16)

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3.2.3 Criterion XT Bis-Tris Gels
Criterion XT Bis-Tris gels are based on a Bis-Tris HCl buffer system (pH 6.4) that uses discontinuous
chloride and MES or MOPS ion fronts to form moving boundaries that stack and separate denatured
proteins by size. This chemistry of XT Bis-Tris gels allows maximum stability and consistent results with
a shelf life of at least 12 months.
Running XT Bis-Tris gels with either XT MES or XT MOPS denaturing running buffer produces different
migration patterns. A combination of these two running buffers and three XT Bis-Tris gels can generate
up to six different migration patterns for small to midsize proteins.
Gel Composition
Gel buffer Bis-Tris HCl, pH 6.4
Crosslinker 5% C
Stacking gel 4% T, 5% C
Storage buffer Bis-Tris HCl, pH 6.4
Shelf life 12 months at ambient temperature; expiration date is printed on each cassette
Migration charts for protein standards on Criterion Tris-HCl, Criterion TGX, and TGX Stain-Free gels.
Criterion Precast Gels