Applications, Gel documentation, Purity assessment – Bio-Rad Gel Doc™ EZ System User Manual

Page 16: Molecular weight assessment

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Criterion Stain Free

TM

System | Criterion Stain Free System Overview

2

Applications

Image Lab software has been designed to perform automated protocols for
routine gel imaging documentation and analysis.

Gel Documentation

Gel electrophoresis is a common way to separate, identify, and purify proteins.
The Criterion Stain Free System allows users to image and print gels for
documentation in laboratory notebooks, or to export images for publication or
presentations.

Purity Assessment

The relative quantity of a protein band in a given sample is used to assess
purity. This is expressed as the percent of total signal in defined bands, in any
given lane.

Molecular Weight Assessment

SDS-Polyacrylamide gel electrophoresis is used to separate proteins
according to their size. Protein molecular weight standards provide a
reference for estimating the molecular weight of the proteins being tested.

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