Bio-Rad Gel Doc™ EZ System - Manual (Page 67)

Displaying Results Data
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Lane Profile
The Lane Profile shows you a cross section of the selected lane, rotated to 90
degrees. You can select other lanes by clicking on them while the Lane Profile
window is open.
The Lane Profile shows the subtracted background under the blue line, and
the area used for quantification in faint green dots under the red line.
You can change how the bands are identified by choosing from the options in
the drop down list. By default, the bands are labeled with the band number.
S CALE TO FIT GRAPH
You can choose the highest point of the display to define the range of the
graph, to provide the best view of one gel image.
You may want to uncheck the Scale to Fit Graph checkbox, to display the
entire range of possible intensity values (0-4095) in your graph. This allows
valid comparisons between gel images.
Subtracted
Background
Rotated
selected
lane