Overview of the imaging process – Bio-Rad Gel Doc™ EZ System User Manual

Page 17

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Overview of the Imaging Process

3

Overview of the Imaging Process

There are two ways to image your gel on the Criterion Stain Free Imager.
Image Lab must be open on your PC, and the Imager must be on.

You can run the default protocol included with Image Lab, which activates and
images the gel with one touch of a button, or customize a protocol based
upon your exact requirements.

To run a default protocol:

1. Open the Imager door and remove the sample tray.

2. Place your gel on the sample tray and insert it into the Imager until you

feel the magnet grab the tray.

3. Press the green Run button on the front panel of the Imager.

Alternatively, you can press the Run Default Protocol button in the
Quick Start Guide. The default protocol will run automatically. See
“Running the Default Protocol” on page 33 for full information.

To run a custom protocol:

1. Open the Imager door and remove the sample tray.

2. Place your gel on the sample tray and insert it into the Imager.

3. Select “Create Protocol” from the Image Lab Quick Start button, or

“File > New Protocol”. Select the parameters for your protocol. Refer to
“Creating and Running Custom Protocols” on page 34 for full
information.

4. Click the green Run Protocol
button on the Protocol Steps
screen to run the protocol.

5. Depending upon the protocol
you have specified, the software
will control the imager to activate
the sample, analyze an image, then
generate and print a report.

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