Native charge density, Page, Quantitative imaging – Bio-Rad Gel Doc™ EZ System User Manual
Page 88: Relative quantity, Sds-page, Tris, Tryptophan, Uv-b, Uv transilluminator
Criterion Stain Free
TM
System | Glossary
74
Native charge density:
The inherent electrical charge of a protein
without the addition of SDS.
PAGE:
Polyacrylamide gel electrophoresis; an electrophoretic method in
which molecules migrate through a molecular lattice created by polymerized,
cross-linked polyacrylamide.
pl:
Isoelectric point; the pH at which a protein molecule carries no net
charge.
Quantitative Imaging:
Determines the quantity of a protein’s
components, through analysis of the pixel values in a digital image of the
sample.
Relative Quantity:
This is a measurement of a specific band’s
relative quantity to the total of all identified bands within a given lane.
SDS:
Sodium dodecyl sulfate (sodium lauryl sulfate); a denaturant which
provides a negative charge
SDS-PAGE:
Polyacrylamide gel electrophoresis performed with the
denaturant sodium dodecyl sulfate.
Tris:
Tris(hydroxymethyl)aminomethane; a buffer component.
Tryptophan:
An essential amino acid which is a precursor of important
biochemical molecules indoleacetic acid, serotonin, and nicotinic acid.
UV-B:
The range of ultraviolet light used by the Stain Free system.
UV transilluminator:
The part of the Stain Free Imager that
transmits UV light through a sample.