Bio-Rad Gel Doc™ EZ System - Manual (Page 50)

Criterion Stain Free TM System | Designing Protocols
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S TEP 2. I MAGE G EL
E XPOSURE T IME
Image Lab will acquire an image using the optimal exposure time, if you select
the Auto button. This option will ensure the best use of the dynamic range.
Use a longer exposure time to visualize very faint bands. That may cause
saturation (overexposure) of more prominent bands, so they cannot be
quantified. Exposure time can range from 0.001 to 10 seconds. Select the
Timed exposure to override automated imaging, if you want to see a stronger
signal from very faint bands. Whatever time is chosen, you can view the actual
exposure used in the Image Info window later.
H
IGHLIGHT SATURATED PIXELS
Check this box if you would like to see any saturated pixels in red, in order to
show how much of the gel image is saturated. This option can be changed
later on by selecting View > Image Transform.
I MAGE C OLOR
You have seven color choices. The first three are explained below. The
remaining choices supply enough color variation to highlight small differences
in the image data. See page 47 to see the color choices dialog box.
• Gray - gray scale data representation, good for black/white printing
• Stain Free - true color representation of the fluorescent signal
• Coomassie - mimics the color of a Coomassie-stained gel
• False Color
• Spectrum
• Gold-Silver
• Pseudo