Bio-Rad Gel Doc™ EZ System - Manual (Page 53)

Creating and Running Custom Protocols
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E DIT B UTTON
The Edit button opens the Manage Standards dialog box, which allows you to
add other third-party standard samples, and to edit your list.
S
TANDARD
L
ANES
You can choose which lane will display your Standards, by typing lane
numbers, or the words First and Last, in the Standard Lanes field. The format
is xx, xx, xx, ... where xx is the lane number. For example, if you run an 18-well
gel and want your standards in lanes 1, 10 and 18, enter First, 10, and Last.
NOTE: Lane detection works best when standards are placed in
the first and last lanes.
R EGRESSION M ETHODS
To calculate the molecular weight of the unknown bands, a regression model
is used. The software will use the relative front and molecular weight values of
the standard bands to calculate the standard curve. This standard curve is
then used to calculate the values of the unknown bands. The shape of the
standard curve is based on the selected regression method. You have a
choice of four regression methods.
The methods and the minimum number of standard bands required for each
type is shown in the following table.
Regression Method Minimum number of standard bands
Linear (semi-log) 2
Point-to-Point (semi-log) 2
Logistic 5
Cubic Spline 5