Bio-Rad Gel Doc™ EZ System - Manual (Page 60)

Criterion Stain Free TM System | Viewing Results
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Image Transform
Use the Image Transform dialog box to adjust image brightness and contrast,
optimizing the image display. The minimum to maximum range will vary,
according to the light and dark values present in your image.
NOTE: These adjustments do not change the data, only the way
the data is displayed. The human eye cannot see as much range
as is contained in the image.
The Frequency Distribution Histogram shows the total data range in the image
and the amount of data at each point in the range.
The Auto Scale button will determine an optimal setting for your particular
image automatically. The lightest part of the image is set to the minimum
intensity, and the darkest is set to the maximum.
• Use the High slider to determine the darkest value that will appear
in your gel image
• Use the Low slider to determine the lightest value to appear in your
image
• The Gamma slider lightens or darkens your image uniformly
You can also type numerical values in the text boxes next to the sliders.
Clicking anywhere on the slider bars will move the sliders incrementally.
Options:
• Invert image display - inverts dark bands on a light background to
light bands on a dark background, and vice versa