Bio-Rad BioLogic QuadTec™ Detector and Components - Manual (Page 17)

Figure 2-5. Manual Control Screen, Showing the QuadTec Detector
USE WITH THE BIOLOGIC DUOFLOW SYSTEM SYSTEM INSTALLATION AND SETUP
2-6
BioLogic Duo-Flow - <user name> - <project name> - <method name>
File View Utilities Options
Edit
Method
New
Method
New
Run Browser Manual Setup Setup Protocol
wash
load
1
2
Run Notes
Advance
Fraction collector
START
Gradient pump: F-10
Inlet A:
1.00
100
0 Inlet B:
Flow rate: ml/min
%
%
High
limit:
0
1000
Low
limit:
psi
AVR7-3 at port 4 SVT3-2 at port 1 SV5-4 at port 2
P L 2 1
2 1
3 4
STOP START STOP START STOP
Set
Set
Set
psi
Tube number:
ml
ml/mir
Volume left:
Report PostRun Log Settings
Window Help
Mode: System Local Mode:
Lamp
Range
Wavelength Selection
System Local
Start Tube:
End Tube:
Fraction size:
1
5
0.15 ml
128
1.00
nm 280
nm 260
nm 214
nm 405
Flowrate: 0.100
% EGP B 0
% Split 0.00
Rack: Rack 1 (128 tubes)
I
AVR9-8 at port 5
3 7
8 2
6
5
4
I
QuadTec Detector
Deuterium
190 - 370 nm
OFF
Zero Baseline
2.00
100% Buffer B
0 2 4 6 8 10 Minutes AU mS/cm
1.50
1.00
0.50
50
0.00
500.0
0.0
400.0
300.0
200.0
100.0
Fractions
GP-pressure QuadTec-3 (214 nm)
Clear
Traces
Econo Gradient Pump
Mode:
Flow
Direction
System Local
WL1 - 280nm
4.000 AU
WL2 - 260nm
-0.00101 AU
WL3 - 214nm
-0.00100 AU
WL4 - 405nm
-0.00100 AU
Flow Rate EGP %B
0 %B 0.100 ml/min 0%
Split
1.00ml/min 0 %B
Gradient Pump: F-10 UV Conductivity
438 psi -0.00278 AU 379 mS/cm
QuadTec
Econo Gradient
Pump
SIM1/pH SIM1/SIG
1.907 AU 0.00 pH
Clear all trace data, start chromatogram from time = 0