Bio-Rad BioLogic QuadTec™ Detector and Components - Manual (Page 49)

Problem Possible Cause Suggested Solutions
Wavelengths chosen Software options are
in the Setup editor
not configured
detector devices box appropriately.
do not show up on
the Run screen
chromatogram.
No traces are seen Software options are
on the Manual not configured
screen or Run appropriately,
screen or lamp output is low.
chromatogram.
With the BioLogic HR BioLogic HR software
software, no traces
options and QuadTec
are seen in either the Setup menu may not be
Manual screen or configured appropriately.
Run screen
chromatograms.
UV baseline is noisy Lamp output is low,
or unstable. or the flow cell is dirty.
UV baseline shows Air bubbles trapped in
a “zig-zag” or saw- the flow cell is a very
tooth trace. common cause.
TROUBLESHOOTING SYSTEM INSTALLATION AND SETUP
6-2
1. Click on the Run screen Settings button to choose
which traces are visible.
2. Zero the baseline as appropriate.
3. Re-scale the chromatogram traces using the scroll
bars as appropriate.
1. Ensure the correct lamp is installed and that the
lamp is turned on both in the BioLogic Manual
screen QuadTec control panel and in the QuadTec
detector setup menu.
2. Check on the lamp status using the QuadTec
setector setup menu. Replace the lamp if
necessary and ensure the correct Setup
configuration is chosen (see Maintenance chapter).
3. Zero baseline and adjust scales as appropriate.
1. Ensure that the QuadTec detector and lamp are
turned on (hardware and software).
2. Carefully follow the instructions in chapter 3 to
ensure that the analog outputs from the QuadTec
to the SIM-HRs are correct.
3. Ensure that the SIM-HRs are configured correctly
both in the HR software and the QuadTec Setup
menu with regard to voltage input and ranges (see
chapter 3).
4. Ensure that the correct wavelengths are chosen on
the QuadTec faceplate.
5. Check on the Manual screen and Run screen
Settings editor to ensure that the desired traces
are active.
6. Zero the detector from the QuadTec faceplate
1. Check the lamp status as described in the
Maintenance chapter. Replace if necessary.
2. If lamp is OK, clean the flow cell using the
procedure detailed in the Maintenance chapter.
1. Always degas buffers.
2. Inject a few ml of methanol into the flow cell to
clear the bubble.
3. Connect a 40 psi back-pressure device after the
flow cell to prevent outgassing.