Bio-Rad BioLogic QuadTec™ Detector and Components - Manual (Page 44)

5.0 MAINTENANCE
5.1 CHECKING THE STATUS OF THE DEUTERIUM LAMP
The deuterium lamp used with the QuadTec has an extended life time to ensure long-time functionality and
reliable measurements with low noise and baseline drift as well as high sensitivity. The lifetime of the lamp
depends on different factors, like the number of lamp starts, average burning time, and user requirements for
noise and sensitivity.
To check the functionality of the lamp, routinely check the two intensity values “sig” and “ref” found in the
Signal field accessed in the QuadTec Setup menu. The “ref” value refers to the intensity of the light
measured in the reference channel and can be used for checking the quality of the lamp. To check these
values:
1. Replace the flow cell with the dummy flow cell originally shipped with the QuadTec.
2. Wait 10 to 15 minutes for the deuterium lamp to warm up, and then select wavelength 1 = 240,
wavelength 2 = 0.
3. Access the Setup menu Signal field and record the value of “sig” and “ref.”
The expected value with a new deuterium lamp should be in the range of 0.1 to 0.9.
If higher noise levels or decreased sensitivity are observed on working with the QuadTec and these
observations coincide with a ref value of approximately 0.2 or less, a new deuterium lamp should be
installed.
MAINTENANCE SYSTEM INSTALLATION AND SETUP
5-1