Luminex 100 IS Version 2.1 - Manual (Page 14)

Luminex 100 IS Developer Guide to xMAP Technology Version 2.1 x MAP Technology
8 PN 89-00002-00-062 Rev. A
Gating
Determine the gating on the doublet discriminator channel for the
assay during development. The numeric values appear on the left-
hand side of the histogram. Use the numerical gate position
determined in development to set the gate location in the templates.
Gating information may change with a new lot of microspheres.
Each time you receive a new lot of microspheres, re-evaluate them
with the current templates. If gating information changes, create a
new template that is the same as the current template but with a new
version number and new gating information. Gate positions are
dependent upon buffer composition. Any changes made to the
buffer composition in your assay may result in a different
optimal gate location.
Plates
Heated assays: select one of these recommended plates:
•
Polyfiltronics 96-well plate, 200 µL, catalog #7703-1902, black.
• CoStar Thermowell 96 well plate, thin-wall polycarbonate,
model P.
Nonheated assays: select a 96-well plate with an overall height no
greater than 0.75 inches (19 mm). When using uncovered plates,
use black opaque plates if possible to reduce photobleaching of
beads.
Instrument Calibrators
and Controls
The Luminex 100 IS system requires calibration:
•
at least once a month
• if the d cal temperature (difference of current temperature and
the last calibrated—between the yellow arrows on the DD Temp
gauge) changes by more than ±3°C
• if the system is turned off or moved
• if the assay controls are not within the prespecified range
• as needed for troubleshooting.
Always run system controls after calibration and as needed for
troubleshooting.
To minimize carryover, Luminex recommends a wash in between
instrument calibration and instrument control steps.
What to Do Next
Continue with the following Acquisition Detail section to develop
your assays.