Continued – Luminex xPONENT 3.1 Rev 2 (IVD) User Manual

Page 106

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Term

Definition

microparticle

A solid substance with a diameter in the
micrometer range. Often used as a synonym for a
microsphere.

microspheres

Polystyrene spheres with a diameter in the
micrometer range. Also called beads.

multi-analyte

Several assays or tests performed simultaneously
in the same reaction container.

multi-batch

A set of batches to be processed consecutively
on a single plate.

panel

A panel is a grouping of related functions and text
fields on the xPONENT user interface.

pending batch

A batch that has been set up, but not yet run.

photobleaching

The process in which light absorption converts the
fluorochromes on the beads into different
fluorescent or nonfluorescent compounds.
Photobleaching prevents beads from being
properly classified.

plate

A 96-well tray in which analytes and samples are
placed for organized acquisition.

plate heater

An aluminum block used in the Luminex analyzer
to maintain sample temperature.

PMT

Photomultiplier tube, measures the excitation
emission intensity of the reporter dye bound to the
surface of the xMAP microspheres.

probe

See Sample Probe.

protocol

A file containing a sequence of commands and
predetermined settings provided with your assay
kit.

Qual

A value that determines a cutoff or threshold.
This, in conjunction with ranges using the Lum
Qual Formula, Adv Qual Formula, or an edited
range specific for your assay, helps to determine
qualitative results for unknown samples.

qualitative

Pertaining to calculations that determine the
absence or presence of an analyte.

quantitative

Pertaining to calculations that determine the
precise numerical measurement of an analyte.

reagent

A substance used to detect or measure other
substances.

region event

A gated event that exhibits the correct
classification fluorescence values to fall into one
of the bead regions. Region events are used to
calculate the median fluorescence intensity (MFI)
which is the final data generated by the system.
Any other event is excluded from data analysis.

TABLE .

Continued

xPONENT

®

3.1 Rev. 2 Software User Manual

96

For In Vitro Diagnostic Use

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