Luminex xPONENT 3.1 Rev 2 (IVD) - Manual (Page 85)

2. Review the adjustment location for the well that you will use for alignment. Click a well on
the plate image if you want to use a different well. A blue pin notes the well selected for
alignment.
3. Remove the clear plastic shield that covers the sample probe area.
4. In a 96-well microtiter plate where overall height is no more than 0.75 inches (19mm),
place the appropriate alignment tool in the plate:
• For a standard plate with flat bottom wells - stack two of the larger (5.08 mm diameter)
alignment discs into the selected well.
• For a filter bottom plate - stack three of the larger (5.08 mm diameter) alignment discs
into the selected well.
• For a half-volume plate with flat-bottom wells - stack two of the smaller (3.35 mm
diameter) alignment discs into the selected well.
• For a round-bottom (U-bottom) plate - stack two of the smaller (3.35 mm diameter)
alignment discs into the selected well.
• For a plate with conical wells - place one alignment sphere into the selected well.
5. Verify that the microtiter plate is not warped. Warped plates can lead to incorrect probe
height adjustment
6. Click Eject to eject the plate holder. Place the plate on the plate holder with well A1 in the
top left corner.
7. Click Retract to retract the plate holder.
8. Loosen the probe adjustment screw on the probe holder one-third to one-half turn. Pull
the sample probe upward until it touches the top of the adjustment slide. Tighten the
probe adjustment screw.
9. Click Move Probe Down to lower the sample probe.
10. Gently loosen the probe adjustment screw. Push the probe down until it just touches the
top of the alignment discs or sphere.
11. Tighten the probe adjustment screw.
NOTE: Use caution not to move the probe up accidently when tightening the
probe adjustment screw.
12. Click Move Probe Up.
13. Replace the plastic shield that covers the sample probe area.
14. Click Eject to eject the plate holder. Remove the alignment tools from the plate.
System Initialization
xPONENT Software contains a pre-defined startup routine to prepare the analyzer for data
acquisition. This section describes warmup, calibration, and performance verification of the
system.
Warm up the lasers to prepare the optics prior to sample acquisition. The system
automatically begins warming up when you turn power on; however, you will need to use the
Warmup command if the system is idle for four hours or longer. Failure to properly warm up
the lasers will affect assay results and system performance.
• On the System Status bar, click the Laser: button if it is yellow. OR
For In Vitro Diagnostic Use Using the Software
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