Luminex xPONENT 3.1 Rev 2 (IVD) - Manual (Page 14)

xMAP beads are hydrophobic in the aqueous medium provided and will settle if left
undisturbed. Resuspend prior to dispensing.
Do not use this product with strong organic solvents. For information on specific compatibility,
visit the Luminex Technical Support website at
Luminex Technical Support .
Safety Precautions
All samples should be regarded as potentially contaminated and treated as infectious. These
samples should be handled at the Biosafety Level 2 as recommended for any potentially
infectious human serum or blood specimen in the Center for Disease Control/National
Institutes of Health Manual, “Biosafety in Microbiological and Biomedical Laboratories” 1984.
Although beads do not contain hazardous or carcinogenic components at toxic levels, they
may be toxic if swallowed. Contact with acids liberates toxic gases. If product comes in
contact with skin, wash immediately with copious amounts of water. In case of accident, seek
medical advice immediately and show the product label or container to your medical provider.
A Material Safety Data Sheet is available upon request.
WARNING: Reagents may contain sodium azide as a preservative. Sodium
azide may react with lead and copper plumbing to form highly
explosive metal azides. It is also highly toxic and rates a
material safety data sheet (MSDS) health hazard 4. On
disposal, flush drains with a generous amount of cold water to
prevent azide build-up. Consult the manual guideline “Safety
Management No. CDC-22, Decontamination of Laboratory Sink
Drains to remove Azide salts” (Centers for Disease Control,
Atlanta, Georgia, April 30, 1976).
CAUTION: The sheath fluid and the solution in which beads are stored
contain ProClin
® , which may cause an allergic reaction. Use
personal protective equipment, including gloves and safety
glasses.
Bead Concentration
The concentration of beads in an assay is a factor in system speed. If running an xMAP-
based kit, follow the instruction found on the kit’s product insert and use the provided
software protocol.
Repetitive xMAP Bead Measurements
In an xMAP assay, the reporter signal is the result of the assay. Due to small bead size,
xMAP bead suspension exhibits near solution phase reaction kinetics. This means that each
set of xMAP beads used for a particular assay will show a statistically even distribution of
reporter molecules bound to the surface of each bead. During data acquisition, numerous
beads of each set are analyzed and the median statistic is computed for that set by the
software. The more beads of a set measured, the more confidence that can be given for that
particular measurement. If running an xMAP IVD kit, follow the kit’s product insert and use
the provided software protocol.
Classification and Reporter Fluorochromes
Each xMAP bead set is internally dyed with two classification dyes, or three dyes for beads
above 100-plex. The fluorescence signal of these dyes allows for classification of each bead
xPONENT ® 3.1 Rev. 2 Software User Manual
4
For In Vitro Diagnostic Use