Metrohm 761 Compact IC - Manual (Page 43)

2.7 Precolumns and separating columns
761 Compact IC
33
2.7.3 Precolumn glass cartridges with cartridge holder
The precolumn glass cartridge METROSEP Anion Dual 1 (6.1006.030)
is inserted in the 6.2828.010 Precolumn cartridge holder as follows (see
Fig. 13):
1 Insert cartridge
• Remove end fittings 82 from screw caps 83.
• Insert precolumn cartridge 85 in sleeve 84 (mark flow direc-
tion of the column cartridge on sleeve, the column cartridge
should always be operated in the same flow direction).
• Screw both screw caps 83 loosely to sleeve 84 by hand.
• Screw both end fittings 82 into screw caps 83 so that their
capillary ends are seated in the PTFE seals of the cartridge.
• Tighten both screw caps 83 by hand.
2 Connect precolumn
• Provide the column connection capillary 28 mounted on the
injection valve with a PEEK compression fitting 54 (see sec-
tion 2.3.5) and screw it tightly onto end fitting 82 on the inlet
side of the precolumn.
• Cut off as short a piece as possible from PEEK capillary 56
and fit this with two PEEK compression fittings 54 (see sec-
tion 2.3.5).
• Screw prepared capillary 56 to end fitting 82 on the other end
of the precolumn.
3 Rinse precolumn
• Place a beaker beneath the outlet capillary of the precolumn.
• Open software window for manual system control.
• If necessary, modify Flow rate to the value suited for the
inserted separating column and click on <Send to unit> to
send this value to the 761 Compact IC.
• Switch on high-pressure pump ( IC pump ) by clicking <On>
and rinse precolumn with eluent for ca. 10 min.
• Switch off high-pressure pump by clicking <Off> .
The rinsed precolumn can be connected directly to the separating
column instead of by means of a PEEK capillary. Proceed as follows:
• Screw off PEEK capillary from end fitting 82 of the precolumn.
• Screw off end fitting 82 from screw cap 83 of the precolumn.
• Screw connection piece 86 onto screw cap 83.
• Screw screw cap 87 of separating column 81 onto connection
piece 86.