Bio-Rad Bio-Plex Pro™ Wash Stations - Manual (Page 21)

Basic Operation and Maintenance
Bio-Plex Pro and Bio-Plex Pro II Wash Stations 21
Perform priming for both channels using the following procedure:
Repeat for channel 2.
Failure to Prime
If you have a new instrument or it has not been used for a long time the pump may
fail to draw fluid from the liquid bottles. This problem is easily solved.
1. Disconnect the L1 tube from the bottle (leaving it connected to the
instrument).
2. Hold the free end of the tube high and pipette 3 - 4 ml of deionized
water to the tube while performing the prime procedure in channel 1
as described above.
Alternately you may carefully use a syringe to inject 1 - 2 ml of water directly into the
L1 port of the instrument, reconnect the tubing, and run a prime procedure.
PRIME
< > OK
CHANNEL 1
- + EXIT OK
WASTE BOTTLE OK?
< > YES
PRIME SOLUTION OK?
< > YES
Confirm rinsing solution and tube in bottle
Select and press OK for PRIME
Select CHANNEL 1 using the choice keys (< , >)
Select OK and the prime procedure starts
Confirm waste bottle is connected and
sufficiently empty
PRIME
< > OK
PROGRAM
< > OK
Instrument will perform the prime step and will
then be ready to perform wash protocols
VOLUME 30 mL
+ - OK
VOLUME
< > OK
Press OK if you see VOLUME. If you see
TIME, scroll down to VOLUME, then press OK
PROCEDURE
< > OK
Navigate to the Procedure in the primary
menu and press OK
Press OK