Process step: wash, Defining new programs – Bio-Rad Bio-Plex Pro™ Wash Stations User Manual

Page 58

Advertising
background image

Defining New Programs

58 Bio-Plex Pro and Bio-Plex Pro II Wash Stations

Process Step: Wash

This process step is used to aspirate solution out of the wells and simultaneously
dispense and aspirate the solution.

POS: OVERFLOW

- + EXIT OK

CX PX W ASH

- + EXIT OK

VOLUME 300µl

- + EXIT OK

CHANNEL 1

- + EXIT OK

H-SPEED 10 mm/s

- + EXIT OK

FL.RATE 200 µl/s

- + EXIT OK

Select required dispensing position
(OVERFLOW, CUSTOM, MOVE OVER., MOVE
CUST. or BOTTOM).

Select the channel to be used (1 - 4)
depending on instrument configuration.

Select the volume of solution to be used
(50 - 3000 µl in increments of 50 µl)

Select and confirm wash step (W ASH)
CX = Cycle Number
PX = Program Step Number

Select required flow rate (drip to 500µl/sec).

Select required aspirating speed (1 - 20 mm/sec).

After the process step has been defined the initial
display appears.
CX = Cycle Number
PY = Program Step Number incremented by 1
Press OK to define another wash step
or press – or + to select the next program step,
Program End or a new cycle.

CX PY WASH

- + EXIT OK

CROSSW. ASP. NO

- + EXIT OK

Select Yes using – or + to perform aspiration of
wash crosswise or select No to perform normal
aspiration.

ASP. RATE 2

- + EXIT OK

Select required aspirating rate (1 – 3).

TIME 1 s

- + EXIT OK

Select required aspirating time (1 – 20 mm/s).

Advertising