Process step: wash, Defining new programs – Bio-Rad Bio-Plex Pro™ Wash Stations User Manual
Page 58
Defining New Programs
58 Bio-Plex Pro and Bio-Plex Pro II Wash Stations
Process Step: Wash
This process step is used to aspirate solution out of the wells and simultaneously
dispense and aspirate the solution.
POS: OVERFLOW
- + EXIT OK
CX PX W ASH
- + EXIT OK
VOLUME 300µl
- + EXIT OK
CHANNEL 1
- + EXIT OK
H-SPEED 10 mm/s
- + EXIT OK
FL.RATE 200 µl/s
- + EXIT OK
Select required dispensing position
(OVERFLOW, CUSTOM, MOVE OVER., MOVE
CUST. or BOTTOM).
Select the channel to be used (1 - 4)
depending on instrument configuration.
Select the volume of solution to be used
(50 - 3000 µl in increments of 50 µl)
Select and confirm wash step (W ASH)
CX = Cycle Number
PX = Program Step Number
Select required flow rate (drip to 500µl/sec).
Select required aspirating speed (1 - 20 mm/sec).
After the process step has been defined the initial
display appears.
CX = Cycle Number
PY = Program Step Number incremented by 1
Press OK to define another wash step
or press – or + to select the next program step,
Program End or a new cycle.
CX PY WASH
- + EXIT OK
CROSSW. ASP. NO
- + EXIT OK
Select Yes using – or + to perform aspiration of
wash crosswise or select No to perform normal
aspiration.
ASP. RATE 2
- + EXIT OK
Select required aspirating rate (1 – 3).
TIME 1 s
- + EXIT OK
Select required aspirating time (1 – 20 mm/s).