Bio-Rad Bio-Plex Pro™ Wash Stations - Manual (Page 32)

About the Instrument
32 Bio-Plex Pro and Bio-Plex Pro II Wash Stations
WARNING
When using wash buffers that show a strong tendency to foam, empty the waste
bottle as soon as the foam level has reached the maximum filling level indicated
on the waste bottle. Add a commercially available anti-foaming agent (such as
silicone oil) to the empty waste bottle to reduce foaming.
If foaming continues to be a problem, we recommend switching to a larger waste
bottle (not provided with the Instrument) and to increase the concentration of
anti-foaming agent in the waste bottle. To help facilitate breakdown of the foam
in the waste bottle, carefully swirl the waste bottle from time to time to improve
mixing between foam layer and anti-foaming agent.
Refill anti-foaming agent after emptying waste bottle. For example, when using
the Wacker Anti-Foam Emulsion SE47 (Wacker article code 21640582), the
recommended concentration is 1 ml of antifoaming agent for 1 liter of waste
solution.
Use antifoaming agents and concentrations as recommended by corresponding
manufacturers.
Intended Use
The Bio-Plex Pro and Bio-Plex Pro II are instruments for strip-wise processing of
microplates in the 96-well format. The instrument is designed for professional use
only and should only be operated by trained personnel.
Caution
The wash results obtained with the Instrument are influenced by the
correct use of the instrument, according to the instructions given in this
document, as well as the liquid compounds used (reagents, wash buffer,
chemical components). The instructions for use, storage, and other
handling in connection with samples or reagents must be strictly
followed.
Caution
Before the instrument is installed and switched on, it should be left to
stand for at least three hours, so there is no possibility of condensation
causing a short circuit.
Caution
Before washing procedures are started, make sure that the microplate
position A1 is inserted correctly.