Thermo Fisher Scientific Eutech 2700 Series - Manual (Page 44)

40
another way to analyze DO levels. % saturation is the measured DO level
divided by the greatest amount of oxygen that the water could hold under various
temperature and atmospheric pressure conditions multiplied by 100.
What Is Being Measured?
DO probes respond to the partial pressure of oxygen in liquid or gas being
measured—they measure the “pressure” of oxygen rather than concentration. All
of the oxygen entering the probe is consumed at the cathode where it is
electrochemically reduced to hydroxyl ions producing an electrical current within
the probe:
O
2
+ 2 H
2
O + 4 e -  4 OH –
Since all oxygen entering the probe is chemically consumed, the partial pressure
of oxygen in the electrolyte is zero. Therefore, a partial pressure gradient exists
across the membrane and the rate at which oxygen enters the probe is a function
of the partial pressure of oxygen in the gas or in liquid being measured.
When a probe is placed in air saturated water, the current it produces will not be
affected by the temperature or salinity of the water. The DO concentration in the
water, however, will vary with temperature and salinity. Because it is convenient
to report DO concentration in mg/L or ppm, it is necessary to adjust for
temperature and salinity of the water to get correct readings in these units.
If DO were to be reported in terms of partial pressure or % Saturation, then
temperature and/or salinity compensation for oxygen solubility would not be
necessary. Most probes are temperature compensated—i.e. they convert the
“partial pressure measurement” to mg/L of DO at whatever temperature the water
happens to be at for a given salinity and barometric pressure.
Air Calibration
Understanding the principle of air calibration is easy, once you know that it is
partial pressure that the probe is responding to. When the probe is in air, it is
measuring the partial pressure of oxygen in air. If water is air saturated, then the
partial pressure of oxygen in the water will be the same as it is in air. Therefore,
all you need to know is the temperature of the air in which the probe is placed.
By consulting solubility tables for oxygen at the particular barometric pressure
and salinity of the water being measured, the corresponding concentration (mg/L
or ppm) can be found for air saturated water at the air calibration temperature,
and the meter can be set accordingly. Because most meters are temperature
compensated, they will still give correct readings in mg/L even though the actual
water temperature may be different to the air calibration temperature. Note: The
closer the air calibration temperature is to the water temperature, the more
accurate the calibration.