Bio-Rad Gene Pulser MXcell™ Electroporation System - Manual (Page 10)

Gene Pulser MXcell™ System Manual
1
1 Introduction
Congratulations on the purchase of a Gene Pulser MXcell™ electroporation system! This
instrument is the newest addition to Bio-Rad Laboratories’ powerful electroporation line.
This system is filled with features that will enable you to quickly optimize conditions for
efficient delivery of molecules into most eukaryotic cells, including mammalian cells and
plant protoplasts.
The Gene Pulser MXcell electroporation system is designed to electroporate cells in 96-, 24-
and 12-well electroporation plates.
Overview of the Gene Pulser MXcell™ Electroporation
System
The system includes three components:
• Power module: The power module produces controlled exponential or square
waveform pulses. The unit is capable of producing pulses of up to 500 V in a self-
contained unit requiring no peripheral modules. This ensures delivery of highly
reproducible electroporation conditions to multiwell plates. The system is designed to
allow you to vary any of the following parameters: waveform, resistance, voltage,
capacitance, pulse duration, and number of pulses.
• Plate chamber: The plate chamber holds a variety of multi-well electroporation plates
to provide maximum flexibility.
• Multi-well electroporation plates: The electroporation plates come in three formats:
12, 24, and 96-well. When using the 96-well plate or 24-well plates, 24 different