Bio-Rad Gene Pulser MXcell™ Electroporation System - Manual (Page 31)

Gene Pulser MXcell™ System Manual | Program and Run the System
22
5. Select a waveform ( EXP or SQR).
Press the arrow keys to select a waveform. Then enter the values for each parameter.
Once entered, press
ENTER to confirm the selection.
NOTE: The P in the lower right corner indicates that the required parameters are
complete, and the PULSE button is ready.
6. (Optional) Select another well set.
Press, and hold the up and down arrow keys to select another well set in the plate.
Enter all the parameters for each well set in the run.
7. (Optional) Press the PULSE button to electroporate the sample.
TIP: Once you start a protocol, you can stop the experiment if needed. To stop a
protocol, press and hold the
PULSE button. When the protocol stops, the screen
displays the last pulse (see “Last Pulse Option” on page 26).
Gradient Protocol Option
The Gradient Protocol allows you to specify values that will be used to automatically
generate a gradient of settings across all the wells of a plate. This protocol is a quick way to
optimize conditions for your specific cell type.
NOTE: Gradient protocols run only on 24- and 96-well electroporation plates.
To set up a gradient protocol, first select the part of the plate to apply the gradient. Follow
the instructions in the following sections to set up a gradient protocol in a plate:
• GRADIENT: Run a gradient protocol through a well set or the whole plate (page 23)
• QUADRANT: Run a gradient protocol through a quarter of the wells in a plate (page 23).
Figure 10. Quadrants in 96-well and 24-well plates. A quadrant is one quarter of the
wells in the plate.
The median voltage entered is applied to the mid point of the well set. In a whole plate, this
corresponds to well set EFGH1, in a quadrant, it is either ABCD4, ABCD10, EFGH4, or
EFGH10. Wells to the left of the percent value entered decrease by the percentage entered,
while wells to the right of the percentvalue entered increase by the percent. Upon
completion of the run, values for each parameter can be obtained by using the right arrow
key.
96-well plate 24-well plate