Well set protocols, 96 well/ exp,vgrad/ cgrad, 96 well/ sqr,vgrad, dgrad – Bio-Rad Gene Pulser MXcell™ Electroporation System User Manual
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Well
Set
Protocols
37
Well Set Protocols
Well set protocols include parameters that vary in each well of a 96-well plate. The protocol
includes the following well sets.
96 well/ Exp,Vgrad/ Cgrad
Table 10 shows the parameters that vary in each well of a 96-well plate. The protocol
includes the following well sets:
•
Well sets ABCD 1–12: Exponential wave, voltage gradient (
ΔV), 350 μF, 1000 Ω
•
Well sets EFGH 1-12: Exponential wave, current gradient (
ΔC), 250 V, 1000 Ω
96 well/ Sqr,Vgrad, Dgrad
Table 10 shows the parameters that vary in each well set of a 96-well plate. The protocol
includes the following well sets:
•
Well sets ABCD 1–12: Square wave, voltage gradient (
ΔV), 2000 μF, 1000 Ω, 20 ms
•
Well sets EFGH 1-12: Square wave, pulse duration gradient (
ΔD) , 250 V, 2000 μF,
1000
Ω
Table 11. 96well/ Exp, Vgrad/Cgrad conditions that vary across the plate.
1
2
3
4
5
6
7
8
9
10
11
12
A
ΔV
(V)
100
100
100
200
200
200
300
300
300
400
400
400
B
C
D
E
ΔC
(μF)
200
200
200
350
350
350
500
500
500
1000
1000
1000
F
G
H
Table 12. 96 well/ Sqr,VgradDgrad conditions that vary across the plate.
1
2
3
4
5
6
7
8
9
10
11
12
A
ΔV
(V)
100
100
100
200
200
200
300
300
300
400
400
400
B
C
D
E
ΔD
(ms)
10
10
10
15
15
15
20
20
20
30
30
30
F
G
H