Bio-Rad Gene Pulser MXcell™ Electroporation System - Manual (Page 47)

Gene Pulser MXcell™ System Manual | Pre-Set Protocols
38
Mixed Protocols
The following protocols include a mix of protocols with a variety of well sets, waveforms,
and plate sizes.
Opt 96 well/ Exp,Sqr
Table 13 shows the parameters that vary in well sets for a 96-well plate. The protocol varies
in a wide range of starting conditions for exponential and square waveforms.
Opt 24 well/ Exp,Sqr
These are parameters that vary in each well for a 24-well plate. Except for the larger well
size, the protocol is the same as “Opt 96 well/ Exp,Sqr” on page 38.
Table 13. Opt 96 well/ Exp, Sqr conditions that vary across the plate.
1 2 3 4 5 6 7 8 9 10 11 12
E
X
P
A 150 V
350
μF 200 V
350
μF 250 V
350
μF 300 V
350
μF 350 V
350
μF 450 V
350
μF 250 V
200
μF 250 V
250
μF 250 V
350
μF 250 V
500
μF 250 V
750
μF 250 V
1000
μF
B
C
D
S
Q
R
E 150 V
20 ms 200 V
20 ms 250 V
20 ms 300 V
20 ms 350 V
20 ms 450 V
20 ms 250 V
5 ms 250 V
10 ms 250 V
15 ms 250 V
20 ms 250 V
25 ms 250 V
30 ms
F
G
H