Bio-Rad Gene Pulser MXcell™ Electroporation System - Manual (Page 21)

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12
Well Sets and Quadrants in Electroporation Plates
The Gene Pulser electroporation plates are available in three different formats: 96-well, 24-
well and 12-well. Table 5 shows the recommended cell concentration and volume for each
well in a plate:
For more information about how to electroporate mammalian cells, see “Preparation of
Mammalian Cells” on page 15.
Well sets and quadrants divide the electroporation plates into functional units. You have the
option of running a different protocol in each different well set or quadrant. Each plate
format is divided according to these definitions:
• Well set: A group of wells within a plate
• Quadrant: All the wells or well sets in one quarter of a plate
Well sets
A well set is a group of four wells in a column of a 96-well plate in which programmed
electroporation conditions are delivered simultaneously. Well sets can assist you in
performing the following:
• Replicating experiments by using the same type of cells and same protocol
• Testing different variables under identical electroporation conditions by putting
different experiments (molecules or cells) in different well sets. For example,
deliver different siRNAs into the same cell line.
In a 96-well plate, each well set is composed of 4 adjacent wells in a column. For example
column 1 with rows A, B, C, and D is one well set (Figure 8):
Figure 8. Well sets in a 96-well plate.
In Figure 8, the shaded and unshaded areas represent sets of wells that are grouped
together in a well set. During a run, the parameters entered for each well set are
simultaneously delivered to all the wells in that set.
Table 5. Electroporator plate formats.
Plate format Cell concentration Volume Number of well sets
96-well 1 x 10 5 to 2 x 10 6 100-200 ul 24
24-well 5 x 10 5 to 8 x 10 6 500-800 ul 24
12-well 1 x 10 6 to 1.5 x 10 7 1.0-1.5 ml 12
One well set